In-frame deletion of Escherichia coli essential genes in complex regulon
may pride peach tree A conditional knockout-rescue system was developed to construct an in-frame deletion strain of Escherichia coli essential genes.The target was flanked with marker genes and FRT (FLP recognition target) sites, and a plasmid containing arabinose-induced FLP recombinase was transformed.After arabinose induction, cells could surviv